Acquisition of useful sero-diagnostic autoantibodies using the same patients’sera and tumor tissues

抄録

Cancer tissues are comprised of various components including tumor cells and the surrounding tumorstroma, which consists of the extracellular matrix and inflammatory cells. Since the tumorstroma plays critical roles in tumor development, investigation of the tumor stroma in addition totumor cells is important to identify useful tumor-associated markers. To discover novel and usefulsero-diagnostic markers, a comparative study of tumor-associated autoantibodies (AAbs) in serafrom lung adenocarcinoma (AC) patients was investigated by two-dimensional immunoblottingwith AC cell lines or each autologous AC tissues. Autoantigens identified from tissue and cell linesamples comprised 58 (45 antigens) and 53 spots (41 antigens), respectively. Thirty-six proteinsincluding Transforming growth factor-beta-induced protein ig-h3 (BIGH3) and Hyaluronan andproteoglycan link protein 1 (HAPLN1) were detected only from tissues, 32 proteins only from celllines, and 9 proteins from both. BIGH3 and HAPLN1 expressions were confirmed in the tumorstroma, but not in AC cell lines by immunostaining and immunoblotting. These data suggest thatautologous tumor tissue and serum are important to coincidently detect AAbs derived from the tumorstroma in addition to tumor cells.