Myelin-associated glycoprotein (MAG): Expression on the surface of human natural killer cells

抄録

The characterization of mononuclear cells in human peripheral blood stained by anti-myelin-associated glycoprotein (MAG) antiserum was examined in a two-color immunofluorescence study and by rosetting with K-562 cells, the target cells of human natural killer cells. Human mononuclear cells consisting of more than 95% of MAG positive cells were also stained with Leu 7, a monoclonal antibody against human natural killer cells, but only 50 to 90 % of Leu 7 positive cells were stained with anti-MAG antiserum. The cells that expressed T cell antigen or monocyte antigen were also stained with anti-MAG antiserum. The percentage of MAG positive cells in OKMI positive cells (monocytes) was larger than in OKT3 positive cells (T lymphocytes). The percentage of MAG positive cells in B cells was less than 5% Human mononuclear cells conjugated to K-562 cells were stained by Leu 7 and also by anti-MAG antiserum. These results indicate that MAG positive cells are closely related to human natural killer cells.