THREE ISOFORMS OF FASCIN PURIFIED FROM STARFISH GAMETES

Abstract

Starfish egg fascin was purified by the method previously established for purifying sperm fascin. Both sperm and egg fascins are composed of three isoforms separated by isoelectrophoresis with pI values of 6.0 (α), 6.12 (β), and 6.25 (y). Some differences were detected in the ratios of the amount of the isoforms between sperm and egg fascins. α-Isoform had less affinity for actin filaments than β- or y-isoform.