IMMUNOHISTOCHEMICAL STUDY ON THE DEVELOPMENT OF GASTRIN CELLS IN THE STOMACH, DUODENUM AND PANCREAS OF THE RAT

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With the use of anti-human G17 I serum (R-1301), antiserum R-1301 previously absorbed with pentagastrin (R-1301-G5), and anti-human G34[1-15] serum (R-2703), at least two subpopulations of gastrin-like immunoreactive cells were demonstrated in the pyloric mucosa of the adult rat. The majority of cells are reactive with all the antisera (typical gastrin-like immunoreactivity), but some cells react only with R-1301 (C-terminal immunoreactivity). A few cells of either subpopulation are present in the duodenum, but none in the pancreas of the adult rat. In the pyloric mucosa, typical gastrin-like as well as C-terminal immunoreactive cells first appear on day 20 of gestation, and gradually increase in number during postnatal development. In the duodenum and pancreas, C-terminal and typical gastrin-like immunoreactive cells first appear on day 18 and day 20 of gastation, respectively. In the duodenum, few remain during development. In the pancreas, typical gastrin-like and C-terminal immunoreactive cells disappear on postnatal day4 and day7, respectively. Extrapyloric gastrin cells in perinatal rats are mostly only C-terminal immunoreactive.