IMMUNOCYTOCHEMICAL LOCALIZATION OF GROWTH HORMONE-RELEASING FACTOR IN THE MONKEY (Macaca irus) HYPOTHALAMUS AND MEDIAN EMINENCE

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Using antisera against growth hormone-releasing factor, cell bodies and axon processes were identified in the hypothalamus and the median eminence of the monkey Macaca irus. Cell bodies were located mainly in the arcuate nucleus and along the third ventricle wall, and occasionally in the ventromedial nucleus. Irnmunostained axons were also observed in the dorsolateral hypothalamus. No immunostained cell bodies were identified in the hypothalamic magnocellular nuclei (paraventricular, supraoptic and suprachiasmatic nuclei). In the median eminence, bundles of fibers were present in the external layer. These cell bodies and fibers did not react with antisera against neurophysin, somatostatin, gonadotropin-releasing hormone, and corticotropin-releasing factor.