Localization of connectin and nebulin in chicken breast muscle by immunoelectron microscopy

Abstract

In chicken breast muscle myofibrils treated with antiserum against connectin, two distinct stripes were seen in the A-I junction region and less clear stripes in A bands. Anti-nebulin antiserum stained both sides of the Z line, approximately 120 nm apart. Deposits of gold-labeled antibodies against connectin were scattered around the A-I junction region. Gold-labeled antibodies against nebulin were deposited in lines approximately 120 nm apart from the Z line.