Abstract
In mammalian cells, especially in polarized cells, the Golgi apparatus plays a crucial role in the instracellular sorting and directional-transport of proteins and lipids. The dynamic nature of the apparatus is underscored by the cell cycle-dependent redistribustion and coalesce near the restricted pericentriolar and apical locarlzation in polarized cells. Cytoskeletons might be involved in the dynamic nature, the characteristic shape and the intracellular position of the Golgi apparatus. However, the precise mechanisms of the Golgi-positioning and of the maintenance of its shape in polarized cells have not been clarified yet. To address this problem, we have developed “semi-intact cell” system to study the molecular mechanisms of the “topo-biogenesis” of the Golgi apparatus in polarized MDCK cells.
