Fluorescence Correlation Spectroscopy as an Analytical Tool of Enzymatic Reactions in the Single Molecule Level

Abstract

Fluorescence correlation spectroscopy (FCS) method has been attractive as an analytical tool, particularly in biophysics and biochemistry, with the recent developments of the method. FCS with confocal optics of microscope and real time correlator equipment achieves analysis of fluorescence species in single molecule level from a very sample area (<1 mm) and volume (~fl) in almost real time (sec ~min). An application to the analysis of enzymatic reaction in solution with FCS is demonstrated as a basis of the analysis to tackle the problems in vivo. The concept of FCS and future view are also discussed.