Thermodynamic Analyses of Specific and Non-Specific DNA Recognition Mechanisms by Transcriptional Factor c-Myb

Abstract

The thermodynamics of the interaction of the c-Myb DNA-binding domain(R2R3) and its target DNA have been analyzed using isothermal titration calorimetry(ITC) and protein engineering. The observed thermodynamic parameters gave several new insights into the DNA recognition mechanism, such as the electrostatic contributions, the local folding in the linker region between R2 and R3, and the thermodynamic differences between specific and nonspecific protein-DNA interactions.