Abstract
Recent progress in single molecule imaging techniques has made it possible to visualize individual protein molecules at work even in living cells. Using this technique, we can analyze kinetics of molecular reactions both in living cells and reconstitution systems. Here, introducing two reactions carried out by epidermal growth factor receptor (EGFR), we show examples of single-molecule kinetic analysis. Single-molecule kinetic analysis allows us to obtain reaction parameters in complicated living organisms. Also, it is revealing complex reaction behavior of proteins both in vitro and in vivo.
