Abstract
The development of stopped-flow technique in recent years has enabled the biochemists to study the kinetics and mechanisms of the fast conformation change of biopolymers in solution. This article outlines the experimental technique of the stopped-flow method and areas in which it has been used. Two applications to problems that are of biological interest are discussed in detail; the one is the application to the studies on the unwinding-rewinding reaction of poly nucleic acid, and the other is the unfolding-refolding reactions of proteins.
