Abstract
Recent progress in direct observation of surface monolayer at the air-water interface by fluorescence microscopy is reviewed in this article. Dynamic process of crystallization in two-dimensional molecular film is directly observed by a epifluorescence microscope. Crystallization process and morphological properties are largely dependent on the chemical structure of monolayer forming amphiphiles. Electrostatic interaction at the interface between charged monolayer and counter charged polyelectrolyte dissolved in water subphase strongly affects crystal-lization process of the monolayer. Some enzymatic reactions at the air-water interface can be visualized as morphological change of phospholipid monolayers.
