Biophysics and Physicobiology
Regular Article
Demonstration of correlative atomic force and transmission electron microscopy using actin cytoskeleton
Yutaro YamadaHiroki KonnoKatsuya Shimabukuro
Author information
JOURNALS FREE ACCESS

Volume 14 (2017) Pages 111-117

Details
Download PDF (3017K) Contact us
Abstract

In this study, we present a new technique called correlative atomic force and transmission electron microscopy (correlative AFM/TEM) in which a targeted region of a sample can be observed under AFM and TEM. The ultimate goal of developing this new technique is to provide a technical platform to expand the fields of AFM application to complex biological systems such as cell extracts. Recent advances in the time resolution of AFM have enabled detailed observation of the dynamic nature of biomolecules. However, specifying molecular species, by AFM alone, remains a challenge. Here, we demonstrate correlative AFM/TEM, using actin filaments as a test sample, and further show that immuno-electron microscopy (immuno-EM), to specify molecules, can be integrated into this technique. Therefore, it is now possible to specify molecules, captured under AFM, by subsequent observation using immuno-EM. In conclusion, correlative AFM/TEM can be a versatile method to investigate complex biological systems at the molecular level.

Information related to the author
© 2017 THE BIOPHYSICAL SOCIETY OF JAPAN
Previous article Next article

Recently visited articles
Predecessor

BIOPHYSICS

feedback
Top