BIOPHYSICS
Online ISSN : 1349-2942
ISSN-L : 1349-2942
Regular Article
Lever arm extension of myosin VI is unnecessary for the adjacent binding state
Keigo IkezakiTomotaka KomoriYoshiyuki AraiToshio Yanagida
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JOURNAL FREE ACCESS
Supplementary material

2015 Volume 11 Pages 47-53

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Abstract

Myosin VI is a processive myosin that has a unique stepping motion, which includes three kinds of steps: a large forward step, a small forward step and a backward step. Recently, we proposed the parallel lever arms model to explain the adjacent binding state, which is necessary for the unique motion. In this model, both lever arms are directed the same direction. However, experimental evidence has not refuted the possibility that the adjacent binding state emerges from myosin VI folding its lever arm extension (LAE). To clarify this issue, we constructed a myosin VI/V chimera that replaces the myosin VI LAE with the IQ3-6 domains of the myosin V lever arm, which cannot fold, and performed single molecule imaging. Our chimera showed the same stepping patterns as myosin VI, indicating the LAE is not responsible for the adjacent binding state.

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© 2015 THE BIOPHYSICAL SOCIETY OF JAPAN
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