The Use of RAPD Markers in Lablab Bean (Lablab purpureus (L.) Sweet) Phylogeny

Abstract

The phylogenetic relationships among 102 lablab bean accessions were investigated using RAPD markers. One hundred and one RAPD fragments were generated using 11 single 12 mer primers of arbitrary nucleotide sequences. Two primers, CMN-A22 and CMN-A31 generated robust and easily interpretable markers between wild and cultivated accessions. A dendrogram constructed based on genetic similarity clearly separated wild type accessions from cultivated accessions. Small values of genetic dissimilarity between and among wild and cultivated accessions and diversity of cultivated accessions without relating to geographical distribution suggest the short history of breeding works in the crop.