Effect of Azospirillum Inoculation, Nitrogen Fertilization and Moisture Regime on Grain Yield and Nutrient Content of Rice

抄録

The effect of Azospirillum lipoferum inoculation of seed and seedling of rice and different levels of nitrogen fertilization (0, 45 and 90 kg N/ha) were studied under two types of soil moisture regimes (80% and 100% field capacity) in pot under controlled conditions. Phosphorus, potassium and sulphur were applied at rates of 60 kg/ha, 40 kg/ha and 10 kg/ha respectively, as basal doses. Rice seed and later rice seedlings were inoculated with at least 10⁶ colony-forming units of Azospirillum lipoferum for the inoculated treatments. Colonization of roots by Azospirillum lipoferum was determined by Enzyme-Linked Immunosorbent Assay (ELISA) test. Azospirillum inoculation significantly (P=<0.001) increased the grain yield and nutrient content of rice, and an economic yield was found for the application of 45 kg N/ha with inoculum, but 80% and 100% of the field capacity values did not have any significant effect compared to the inoculation and nitrogen fertilization.