Abstract
During the summer of 2009, a serious rice dwarf disease occurred in several provinces in North Vietnam. The appearance of white to black waxy galls along the veins of the leaves and culms on the diseased plants suggested the infection of a fijivirus like rice black-streaked dwarf virus (RBSDV) or a related virus, namely southern rice black streaked dwarf virus (SRBSDV) = rice blacked streaked virus 2 (RBSDV2), which was recently identified in South China in 2008. The universal primers specific for these viruses were designed based on the conserved regions of the S10 segment. RT-PCR tests on typical samples collected from different provinces gave specific products using these primers. Four RT-PCR products amplified from diseased plants that are representative for the four North Vietnam provinces, Nghe An, Thanh Hoa, Nam Dinh, and Son La, were directly sequenced. Blast searches and sequence analyses revealed that they showed all of the isolates of SRBSDV.
