Bulletin of the Institute of Tropical Agriculture, Kyushu University
Online ISSN : 1881-4212
Print ISSN : 0915-499X
ISSN-L : 0915-499X
BITA 34-1
Chemotaxonomic characterization of fungal genera, causal pathogens of rice seedling diseases, using fatty acid analysis
M. MatsumotoS. S. Aye
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2011 Volume 34 Issue 1 Pages 1-14

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Abstract

Rice seedling blight is an important disease caused by a complex of fungi including to the fungal genera of Fusarium, Pythium and Rhizopus species. These were consisting of F. roseum and F. solani, P. graminicola, P. spinosum, P. irregulare and P. sylvaticum, R. chinensis, R. oryzae and R. javanicus. A modified MIDI method was used for extraction of fatty acids and fatty acid methyl ester (FAME) profiles were characterized relating to the rice seedling pathogens. Total 83 isolates were used in this experiment. All tested isolates were subjected by the principal component analysis and cluster analysis based on the cellular fatty acid compositions to generate qualitative and quantitative variations. A total of 14 fatty acids were detected and FAME profiles showed quantitative and qualitative variations within different species levels of belonging to same genus. In this experiment, the possibility of utilization as the chemotaxonomic tools based on FAME profiles was discussed.

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© 2011 Institute of Tropical Agriculture, Kyushu University
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