Biological control of leaf blight of wheat caused by Bipolaris sorokinian

抄録

Five different plant extracts including neem (Azadirachta indica), mehedi (Lawsonia alba), garlic clove (Allium sativum), rhizome of ginger (Zingiber officinales), seeds of black cumin (Nigella sativa), and BAU-Biofungicide (a Trichoderma based preparation) were used to evaluate the performance or effectiveness of those biological control agent on Bipolaris leaf blight of wheat and related pathogen (Bipolaris sorokiniana). Pathogenic reaction was observed in Bipolaris sorokiniana against different treatments by detached leaf method where leaf spot size was minimum (4.5mm) with BAU-Biofungicide and maximum leaf spot size (32.5mm) with control. Effect of seed treatment on wheat plant was evaluated by rolled paper towel method and BAU-Biofungicide, extracts of garlic clove and neem leaf at the value of 13%, 12% and 10%, respectively higher normal seedlings and BAU-Biofungicide also resulted 26.6% higher vigour index over control. Multiplication effect (seed treatment plus foliar spray of same treatment) of different treatments was examined to determine the efficacy for disease control. In pot and field experiments, though Bavistin and Tilt were most effective however, BAU-Biofungicide and extract of garlic clove were superior compared with the treatments used for controlling leaf blight of wheat. Multiplication effect of seed treatment plus foliar spray showed superior effect by BAU-Biofungicide including higher 1000-grain weight (43.92g) and grain yield (2.75 t/ha). Seed treatment with Bavistin and foliar spray with Tilt showed 1000-grain weight and grain yield by 47.12g and 3.0 t/ha, respectively. Our data also revealed that BAU-Biofungicide increased grain yield (29.87%) over control.