Abstract
Two large native worms are an Amynthas from Kumejima, Okinawa and a Metaphire from Lake Biwa, Shiga-ken both supported by mtDNA COI barcodes, and two new species are described. New 2016 Okinawa exotic reports are of small American lumbricid Bimastos parvus (Eisen, 1874) and confirming of Asian megascolecid Pithemera bicincta (Perrier, 1875).