THE BULLETIN OF NATIONAL INSTITUTE of TECHNOLOGY, KISARAZU COLLEGE
Online ISSN : 2188-921X
Print ISSN : 2188-9201
ISSN-L : 0285-7901
Laboratory Studies of in vivo Fluorescence of Chlorophyll a of a Leaf
Kunio TAKAHASHIShinji URUMATokihisa NAKAMURA
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RESEARCH REPORT / TECHNICAL REPORT FREE ACCESS

1983 Volume 16 Pages 53-56

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Abstract
The distribution of chlorophyll a involved in leaves of spinach, camellia and hibiscus is investigated by studing the fluorescence (685nm) intensity of in vivo chlorophyll a. In vivo chlorophyll a is excited by T. E. nitrogen laser radiation at 337.1 nm and organic dye laser radiation at 420nm. The intensity of the fluorescence is proportional to the amount of chlorophyll a (50μg/l〜10mg/l). The fluorescence intensity increases with the growth of plants and the back of the leaf has an excess of chlorophyll a compared with the surface. Experimental technique and result are discussed in detail.
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© 1983 National Institute of Technology, Kisarazu College
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