Abstract
The distribution of chlorophyll a involved in leaves of spinach, camellia and hibiscus is investigated by studing the fluorescence (685nm) intensity of in vivo chlorophyll a. In vivo chlorophyll a is excited by T. E. nitrogen laser radiation at 337.1 nm and organic dye laser radiation at 420nm. The intensity of the fluorescence is proportional to the amount of chlorophyll a (50μg/l〜10mg/l). The fluorescence intensity increases with the growth of plants and the back of the leaf has an excess of chlorophyll a compared with the surface. Experimental technique and result are discussed in detail.