Fluorometric High Performance Liquid Chromatographic Analysis of Prostaglandin (PG) Metabolites : Application to the Catabolism of PGE₂ and PGF_2α in Rat and Rabbit Kidney Homogenates

Abstract

A previously reported fluorometric high performance liquid chromatographic procedure for separation of 9-anthryldiazomethane (ADAM) derivatives of prostaglandins (PG's) was applied to examine catabolic products of PG's, such as 15-keto-PGE₂, 13, 14-dihydro-15-keto-PGE₂, 15-keto-PGF_2α, and 13, 14-dihydro-15-keto-PGF_2α. ADAM derivatives of these metabolites were separated in 30 min on an ODS column with 65% acetonitrile. By this method catabolism of PGE₂ and PGF_2α in kidney homogenates of rats and rabbits was analyzed. Homogenates of medulla and cortex of rat kidney catabolized PGE₂ and PGF_2α to 15-keto-PG's. Whereas the homogenate of rabbit kidney cortex yielded 13, 14-dihydro-15-keto-PGE₂, that of medulla did not. The conversion of PGF_2α to PGE₂ was shown to occur in homogenates of rabbit kidney cortex as well as rat kidney medulla and cortex.