Biological and Pharmaceutical Bulletin

Featured article

Volume 41 (2018) Issue 10 Pages 1567-1573

Characteristics of Bone Strength and Metabolism in Type 2 Diabetic Model Nagoya Shibata Yasuda Mice

Hiroaki Tanaka, Toshihiro Miura, Takenori Yamashita, Misao Yoneda, Satoshi Takagi

We evaluated the suitability of Nagoya Shibata Yasuda (NSY) mice as an animal model for examining the influence of a glucose metabolism disorder on bone integrity, using Institute of Cancer Research (ICR) mice as controls. We selected six NSY and ICR mice each that were matched for weight, and measured serum glucose levels, serum insulin levels, and conducted an oral glucose tolerance test. Histological sections of the femurs of both mouse lines were prepared, and the bone strength, mass, and microstructure of the femur were compared, along with bone metabolism. Serum glucose levels were significantly higher in the NSY mice than in the control mice, but body weight and serum insulin levels did not differ between the groups. Bone mass, microstructure, and strength of the femur, and bone metabolism were lower in the NSY mice than in the control mice. In the cortical bone of the femur in the NSY mice, several parts were not stained with eosin, demonstrating a strong negative correlation between serum glucose levels and bone mineral density; however, there was a negative correlation between serum glucose levels and bone metabolic markers. The bone turnover rate in the NSY mice was decreased by hyperglycemia, resulting in a thinner and shorter femur, reduced cortical and trabecular areas, and lower bone mass compared to those of the control mice. Collectively, these results suggest deteriorated bone strength of the femur in NSY mice, serving as a useful model for studying the link between glucose metabolism and bone integrity.

Editor’s picks

Type 2 diabetes mellitus (T2DM) animal models are often used in basic research on diabetic osteoporosis. The article by Tanaka et al. elucidated hyperglycemia contributed to a decrease in bone metabolism turnover in the T2DM animal model NSY mice, resulting in a thinner and shorter femur, lower cortical and trabecular area, and lower bone mass. Their results suggest that these effects contribute to the deteriorated bone strength of the femur in NSY mice, and the NSY mouse could serve as an appropriate T2DM animal model for examining the specific effect of hyperglycemia on bone integrity, using the ICR mouse as a control.

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2018 Volume 41 Issue 10

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