Heterocycles Related to Nucleotides. XI. An Organomercurial Column Chromatography for Thiouridine

Abstract

By the organomercurial column : chromagel or Sephadex coupled to p-aminophenylmercury chloride, 4- and 2-thiouridine were selectively separated from the mixtures with major nucleotides. Oligonucleotides containing 4-thiouridilic acid were also separated from the ribonuclease-T₁ digests of unfractionated transfer ribonucleic acid of E. coli.