PRODUCTION OF ANTIBODY TO APROTININ AND LOCATION OF THIS COMPOUND IN BOVINE TISSUE

Abstract

Antibody to aprotinin was easily produced in rabbits by injecting an emulsion of Trasylol and adjuvant, and its specificity was confirmed by double diffusion, immunoelectrophoresis in gel, passive hemagglutination and affinity chromatography of Trasylol. The location of aprotinin in bovine lung and pancreas was determined by an indirect fluorescent antibody technique. The fluorescence observed in the connective tissues was found to be due to the mononuclear cells in these tissues.