METABOLISM OF ISONIAZID AND FREE HYDRAZINE FORMATION IN ISOLATED RAT HEPATOCYTES

MASAHARU HIRATA; YOSHINORI ASO; KUANGYANG HSU; TETSURO TABATA; KENJI MATSUYAMA; ATSUKO NODA; SADAO IGUCHI

doi:10.1248/bpb1978.4.145

Journal of Pharmacobio-Dynamics

Online ISSN : 1881-1353
Print ISSN : 0386-846X
ISSN-L : 0386-846X

METABOLISM OF ISONIAZID AND FREE HYDRAZINE FORMATION IN ISOLATED RAT HEPATOCYTES

MASAHARU HIRATA, YOSHINORI ASO, KUANGYANG HSU, TETSURO TABATA, KENJI MATSUYAMA, ATSUKO NODA, SADAO IGUCHI

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Keywords: mass fragmentography

JOURNAL FREE ACCESS

1981 Volume 4 Issue 2 Pages 145-147

DOI https://doi.org/10.1248/bpb1978.4.145

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Abstract

Isoniazid (INH) was incubated with isolated rat hepatocytes, and the amounts of acetyl-INH, monoacetylhydrazine, diacetylhydrazine and hydrazine formed were determined by gas chromatography-mass spectrometry (GC-MS). Subsequent analyses of kinetic parameters of metabolic pathways and enzymological study of rat liver homogenate revealed that hydrazine was formed preferentially from INH, catalized by soluble hydrolytic enzymes.

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