IMMUNOLOGICAL CROSS-REACTIVITIES OF VARIOUS ANTHRACYCLINE ANTIBIOTICS AGAINST ACLACINOMYCIN A ANTISERA

Abstract

Aclacinomycin A (ACM) antisera were abtained from the rabbits immunized with 4'''-deoxo-4'''-(R)-amino-ACM-or N, N-didemethyl-ACM-bovine serum albumin conjugate, and their immunoreactivites were tested with ACM-related anthracyclines. It was found that the binding ability with the ACM antisera was markedly decreased by the following structural changes in ACM : N, N-didementhylation of the rhodosamine moiety ; 6-O-or 4-O-methylation ; removal of the methoxycarbonyl group at C-10 ; hydroxylation at C-1, C-2 or C-11. It was less affected by some alterations in a side chain at C-9 or by deglycosidation of the terminal mono or disaccharide. The binding of the aglycone (aklavinone) was very weak.