BRAIN DISTRIBUTION OF HYDRAZINE AND ITS GABA ELEVATING EFFECT IN RATS

Abstract

After the intravenous administration of isoniazid (INH ; 0.37mmol/kg) or hydrazine (Hz ; 0.16mmol/kg), the minor metabolite of INH, γ-aminobutyric acid (GABA) and Hz were measured by means of gas chromatograph-mass spectrometer (GC-MS) using deuterium labeled GABA (d₂-GABA) and nitrogen fifteen labeled Hz (¹5N-Hz) as internal standards. In both cases, we successfully detected Hz and GABA in the brain. The brain GABA elevating effect of Hz was much higher than that of INH in spite of low dose.