2016 Volume 65 Issue 12 Pages 737-744
A novel separation method that combines liquid chromatography and electrophoresis in a field of heterogeneous flow velocity, named as field-flow orthogonal electrochromatography (FFOEC), is proposed. In the theory of FFOEC, the difference in the orthogonal electrochromatographic migration velocity of analytes in a planer stationary phase with pressurized flow of controlled-heterogeneous velocity can enhance the analytes separation in the flow direction as a similar manner of field-flow fractionation (FFF). Since the essence of FFOEC is a one-directional separation, the parallel detection system is not indispensable, akin to a conventional HPLC, even though a planer stationary phase is used. The polymer monolithic layer with a heterogeneous monolith density to control the flow velocity was prepared using a modulation of irradiation intensity in photo-initiated polymerization. In FFOEC using the heterogeneous density polymer monolith layer, the separation efficiency of the analytes was successfully enhanced with an increase in the applied voltage due to orthogonal electrophoresis.
