Abstract
A new color reaction of BHT (butylated hydroxy toluene) was reported previously in Part 1, which comprized a reaction of BHT with p-nitrobenzene-diazonium salt after treating BHT with an alcoholic hydrogenchloride reagent. In this report the method was extended to the identification of BHT and BHA in mixtures of phenolic or aminocontaining antioxidant by a simple procedure.
The azo dye which was obtained by the method previously reported could be separated on a sheet of paper treated with aluminum hydroxide or on a small aluminum hydroxide column, using petroleum ether as the developing solvent. The identification limit is 60 μg each of BHT and BHA in 0.3 ml of a sample, but it can be made more sensitive by treating the dye with an alkaline dimethylforamide reagent.
