MICRO HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY OF MUTAGEN, ETHYL N-ETHYL-N-NITROSOCARBAMATE, FORMED FROM URETHANE AND NITRITE

Kazuya KOHASHI; Yuichi KAWAZOE; Tadao HORIUCHI; Yosuke OHKURA

doi:10.2116/bunsekikagaku.31.E41

Kazuya KOHASHI, Yuichi KAWAZOE, Tadao HORIUCHI, Yosuke OHKURA

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Keywords: Micro high-performance liquid chromatography with UV detection, micro high-performance liquid chromatography with mass detection, mutagen, ethyl N-ethyl-N-nitrosocarbamate, ethyl N-nitrosocarbamate

JOURNAL FREE ACCESS

1982 Volume 31 Issue 1 Pages E41-E44

DOI https://doi.org/10.2116/bunsekikagaku.31.E41

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Abstract

The separation of mutagen, ethyl N-ethyl-N-nitrosocarbamate (EENC), from an acidic mixture (pH 1.2) of urethane and sodium nitrite is described, which employs micro high-performance liquid chromatography on a reversed-phase column (LiChrosorb RP-18). Ethyl N-nitrosocarbamate was characterized as an intermediate to EENC.

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