New type valve for flow injection analysis

I. Application to stopped-flow spectrophotometric determination of glucose

Abstract

A new type valve for a stopped-flow flow injection analysis (FIA) was developed. The valve has two characteristic functions. The first is that a sample solution is injected in a “plug flow” into a flow of eluant, and the second is passage change-over of the eluant flow.At the load position, the eluant is recycled from tank to tank and the sample loop is filled with sample solution when the flow in reaction tubing is stopped. At the injection position a sample solution is injected into the flow of the eluant and is sent into reaction tubing. By rotating the valve from a injection position to a load position the reaction flow is stopped in a reaction tubing and the reaction will proceed. Stopped-flow FIA using the new type valve was one of the useful method for determination of glucose.