1988 Volume 37 Issue 2 Pages 109-112
The immobilized adenosine triphosphatase(ATPase) and alkaline phosphatase(ALP) reactors were used with ion chromatography for the simultaneous determination of adenosine triphosphate(ATP) and inorganic ions such as nitrate and sulfate ions. In this system, the immobilized enzyme reactor is attached in front of the injection loop to convert ATP to phosphate ion before the sample is introduced into the eluent. In case of the sample flow rate through the immobilized enzyme reactor 0.1 ml/min, the conversions of ATP to phosphate ion obtained by ATPase and ALP reactors were found to be 68.5% and 17.4%, respectively. When ATPase reactor is used at the sample flow rate 0.1 ml/min, the calibration curve for ATP is linear over the range 7×10-61×10-4 M and relative standard deviation is 1.1%, and the detection limit is 4×10-6 M. The activities of ATPase and ALP reactors were stable for one and three months, respectively.
