1994 Volume 43 Issue 1 Pages 71-74
According to the official method, the 4 pesticides used on golf courses (mecoprop, oxin-copper, simazine, and thiram) which leach into the drinking water supply are to be determined separately. A simple and simultaneous method of determination of these pesticides is described in this paper. Two milliliters of filtered sample was injected into the on-line enrichment column which was a substitute for an injection loop of a sample injector. Back-flushed pesticides, using a pumped mobile phase onto an analytical column, were analyzed. Conditions for HPLC analysis were as follows: analytical column, Shodex RSpak DE-613; extraction column, Merck LiChroCART; mobile phase, 50% aqueous solution of acetonitrile containing 0.5 mM phosphoric acid. Linear calibration curves based on the peak area were obtained in a range of 120 ng, 0.6100 ng, 0.420 ng, and 120 ng for mecoprop, oxin-copper, simazine, and thiram, respectively. Detection limits (S/N=5) were 0.6 ng, 0.2 ng, 0.1 ng, and 0.4 ng for mecoprop, oxin-copper, simazine, and thiram, respectively. The recoveries of the 4 pesticides added to drinking water at the 5 or 10 μg/l level ranged from 93 to 101 %. The proposed method is considered to be applicable for the routine examination of water.
