Abstract
Chromatographic separation of polar dyes was carefully carried out. The enzyme and the alkali-polar dyes were respectively fractionated into three and two major components on Amberlite CG-50 column. The first and the second fractions of the enzyme-polar dye showed chromatographic behavior similar to the corresponding fractions from the alkali-polar dye. The third fraction of enzyme-polar dye seemed to be greatly decreased in quantity in the alkali-polar dye. Each fraction of the alkali-polar dye was purified by ascending paper chromatography. The second fraction was further separated into two components. Their physicochemical properties such as electronic absorption spectra and ionization constants were determined.
