DYE-PROTEIN BINDING IN THE ANIMALS ADMINISTERED WITH 4-DIMETHYLAMINOAZOBENZENE AMINE-N-OXIDE AND THE TOXICOLOGY OF 4-DIMETHYLAMINOAZOBENZENE AMINE-N-OXIDE

抄録

Following previous experiments on the dye-protein binding in in vitro system, the dye-protein binding in in vivo systems is reported, in which 4-dimethylaminoazobenzene amine-N-oxide was administered intragastrically or intramuscularly to rats, mice, and guinea pigs. The binding took place at the site of injection as well as in the liver in the case of intramuscular administration. Amount of the protein-bound dye increased with the dose. LD₅₀ values of 4-dimethylaminoazobenzene amine-N-oxide were compared with that of 4-dimethylaminoazobenzene, using rats and mice, both by intragastric and intraperitoneal administration. The highest toxicity was observed in the intraperitoneal injection of 4-dimethylaminoazobenzene amine-N-oxide. The toxicity decreased to 1/5 (mice) and to 1/15 (rats) in the case of intragastric injection. Addition of 4-dimethylaminoazobenzene amine-N-oxide in the drinking water (0.03%) to rats and mice caused malignant hepatomas in rats, but only adenomatous growth in mouse liver.