INDUCTION OF HEPATOMA AND SYSTEMIC AMYLOIDOSIS IN MICE BY 4-(DIMETHYLAMINO)AZOBENZENE FEEDING

Abstract

The carcinogenic activity of 4-(dimethylamino)azobenzene (DAB) in mice of high spontaneous hepatoma strain, C3H/HeOs, and of low hepatoma strain, C57BL, was studied.
DAB which has been known as being less active carcinogen for mice successfully induced hepatomas with cirrhotic changes in the liver in both strains of mice when the experimental animals were fed 0.06% DAB in animal chow, for as long as 5 months. The induced hepatomas were indistinguishable morphologically from those of spontaneous tumors.
Animals fed 0.06% DAB chow for 1 week to 1 month did not develop hepatoma or liver change but high incidence of systemic amyloidosis was produced only in mice of C57BL strain.