EFFECT OF CHONDROITINSULFATE ON THE GROWTH OF SOLID EHRLICH ASCITES TUMOR AFTER RECHALLENGE AND IN VITRO INCUBATION

Abstract

Examinations were made on the effect of chondroitinsulfate on the growth of Ehrlich ascites tumor after in vitro incubation and a relation between the growth-promoting activity of chondroitinsulfate and suppression of the growth of rechallenged tumor cells.
The tumor cells were suspended in 2% chondroitinsulfate solution or saline and were kept at 37° for 30min. One ml of 2% chondroitinsulfate solution was injected into the subcutaneous space on back of mice, immediately followed by inoculation of tumor cell suspension. It was observed that chondroitinsulfate acted as a growth-stimulating agent in vivo and also in vitro.
One ml of 2% chondroitinsulfate was injected subcutaneously into the back of mice, immediately followed by rechallenge of tumor cells into the same site, on the 1st to 14th day after the 1st challenge into the buttock of mice. On the 8th day after rechallenge, average tumor weight in chondroitinsulfate-treated groups was compared with that in control groups. It was observed that chondroitinsulfate accelerated the growth of tumor both in the case of rechallenge and in challenge only, indicating that chondroitinsulfate counteracts the suppression of the growth of rechallenged tumor cells by the host. Stimulating activity of chondroitinsulfate on the growth of tumor was discussed.