GROWTH INHIBITION OF SARCOMA-180 SOLID TUMOR BY THE CELLS OF REGIONAL LYMPH NODE AND SPLEEN FROM MICE ADMINISTERED WITH YEAST POLYSACCHARIDES

Abstract

Significant growth inhibition on sarcoma-180 solid tumor was demonstrated in the mice which received the regional lymph node cells from mice implanted with the tumor cells and injected with yeast polysaccharide, glucan or mannan. The degree of inhibition was very weak in the mice which received regional lymph node cells from the mice implanted with sarcoma-180 tumor cells without the polysaccharide treatment. On the other hand, the mice implanted with spleen cells from the mice implanted with sarcoma-180 tumor cells regardless of the administration of yeast polysaccharides were found to be significantly resistant to the growth of implanted tumor.
When sheep erythrocytes were administered intravenously into the mice, which were implanted with sarcoma-180 solid tumor and injected with yeast glucan or mannan, the number of antibody-forming cells in the spleen detected by the plaqueformation technique was significantly larger than that in untreated mice.
The conclusion based on the above findings is that both polysaccharides, glucan and mannan, are similar in their behavior to stimulate the antibody response against foreign substances such as sheep erythrocytes and implanted tumor cells.