1977 年 68 巻 1 号 p. 81-87
Solutions of 100mg/kg 1-butylurea and 50mg/kg sodium nitrite were administered daily by a stomach tube to pregnant ACI/N rats from the 13th to 21st day of gestation. Neurogenous tumors were induced in their offspring. The incidence of tumors and mean survival time of rats with nervous tumors were 64% (23/36) and 309 (189∼672) days, respectively. Localization and histological findings of the nervous tumors were similar to those observed previously in rats whose mothers received 1-butyl-1-nitrosourea (BNU) during pregnancy.
Neurogenous tumors did not develop in the offspring of the mothers that received 100mg/kg of 1-butylurea alone in the same manner. In several rats of both groups, a few spontaneous tumors of the testis, pituitary gland, urinary bladder, uterus, and colon were detected.
In vivo formation of BNU in stomach content after intubation of 1-butylurea and sodium nitrite was determined. BNU was detected in the stomach content at levels of 25ppm at 30min and 23ppm at 60min after administration. The concentration of BNU in the stomach content corresponded to 48.3 and 29.2μg/rat, respectively.
