The purpose of the present study was to develop the English Learning Motivation Scale for Veterinary Nursing Students and examine its reliability and validity. In Survey 1, veterinary nursing students (N = 141) completed a questionnaire developed in a preliminary investigation. Exploratory factor analysis revealed that the scale comprised the following 4 factors containing 32 items: “general knowledge and training,” “practical use for veterinary medicine,” “life and study overseas,” and “passive sense of obligation.”The internal consistency of each factor was sufficiently high. In Survey 2, the test-retest reliability coefficients were examined and revealed to be appropriate. Furthermore, the construct validity was tested. The results suggested moderately high convergent and discriminant validity, in accordance with theoretical predictions.