Fault of Ossification and Calcification and Angular Deformities of Long Bones in the Mouse Fetuses Caused by High Doses of Ethane-1-Hydroxy-1, 1-Diphosphonate (EHDP) During Pregnancy

Abstract

Pregnant mice were injected from day 11 to 17 of gestation daily with EHDP in a dose of 200 mg/kg sc. The animals were sacrificed on day 18 of gestation and fetuses were removed surgically from mothers. Fetal skeletons were examined by means of alcian blue and alizarin red S stained preparation and histologically. Using EHDP the weight of the embryo significantly decreased. EHDP produced the shortening of the mineralization zones, a widening of the columnar cartilage zones, inhibition of the formation of the marrow cavity and angular deformities in the long bones.