Abstract
A method of determination of amino acids in tea was investigated by using high pressure liquid chromatography, after prior removal of the impurities from tea extract with milliporefilter.
Amino acids in tea were determined with 3.0-5.0% of coefficients of variation and 100±5% of recovery by this method.
Eighteen amino acids and 4 unknown compounds were detected but the secondary amine such as proline could not be detected and also the complete separation of threonine and serine was not accomplished by this method.