LABORATORY AND CLINICAL STUDIES ON CARBENICILLIN
1970 Volume 18 Issue 2 Pages 133-139
Details
1970 Volume 18 Issue 2 Pages 133-139
A new synthetic penicillin, carbenicillin was studied bacteriologically and clinically. The results obtained were summarized as follows :
1) In a study by the agar plate dilution method on a total of 96 strains of 20 bacterial species, β-hemolytic Streptococci, penicillinase non-producing Staphylococci and Pneumococci were found to be very susceptible to the agent with the MICs of 0.38μg/ml or less. Enterococci, α-hemolytic Streptococci, penicillinase producing Staphylococci, Escherichia, Salmonella, Shigella, Pseudomonas and Proteus-Providence were fairly susceptible with the MICs ranging 50-0.38 pg/ml, while Klebsiella displayed a rather poor susceptibility with the MICs over 100μg/ml.
2) By the thin-layer cylinder-plate method using B. subtilis PCI 219 as a test organism, carbenicillin levels in body fluids were assayable to the lower limit of 0.1 pg/ml. Following a single intramuscular injection of 1, 000 mg of the agent, the blood peak levels were obtained on the lapse of 30 minutes to one hour, with a persistence of effective serum levels over ensuing 6 hours. Bile levels were higher than its blood levels. And the agent was excreted in urine in high concentration and recovered mostly from urine.
3) In order to apply the single-disc method to the sensitivity test of carbenicillin in clinical laboratory, statistical analysis of the dose-response data concerning interrelationship between MIC and diameter of inhibition zone was conducted.
4) Carbenicillin treatment was effective in 3 cases with urinary tract infections caused by Proteus or E. coli.
5) In a case of systemic lupus erythematosus with hypersensitivity to aminobenzyl-penicillin, skin-redness was observed in the region of injection. No side-effect was noticed in other cases of treatment.
