COMBINED EFFECT OF PEPSIN-TREATED HUMAN IMMUNOGLOBULIN AND ANTIBIOTICS IN VITRO

Abstract

Apart from immunological response in vivo when combined dose of antibiotics and immunoglobulin is given, the effect of antibiotics upon in vitro antibacterial activity resulting from the addition of pepsin-treated human immunoglobulin (GGP) was studied on eight strains of gram positive cocci and gram negative bacilli. An enhanced antibacterial activity due to GGP addition was comfirmed in either cases of minimum inhibitory concentration (MIC), number of bacterial colony, and proliferation curve. It was very interesting phenomenon, in particular, that the effect was also seen in each case of Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae, being moderately or more resistant to antibiotics. This phenomenon also was verified through microscopic morphological observation.