GENTAMICIN ENZYME IMMUNOASSAY FOR DETERMINING SISOMICIN SERUM CONCENTRATIONS

Abstract

We evaluated the gentamicin enzyme immunoassay kit (EMIT Qst GM kit) for the determination of sisomicin serum concentrations. We prepared sisomicin calibrators (0, 0.5, 1.0, 2.0, 4.0, 8.0μg/ml) instead of gentamicin calibrators and then obtained a standard curve by use of a reagent for the EMIT Qst GM assay. This standard curve was a good sigmoid curve.
The following results were obtained.
1. The within-run coefficient of variation was less than 10.4%.
2. The between-run coefficient of variation was less than 9.8%.
3. Analytical recoveries of sisomicin from serum spiked with varying concentrations of sisomicin averaged from 96.0-108.0%.
4. The relationship between the modified EMIT Qst method (Y) and high-performance liquid chromatography (X₁) or bioassay (X₂) was studied.
Y=0.751 X₁+0.590, r=0.991; Y=0.958X₂+0.268, r=0.997
We confirmed that the modified EMIT Qst method is a very reliable and useful means of monitoring for clinical sisomicin levels.