1989 Volume 37 Issue 4 Pages 426-432
This work was carried out in order to clarify the antigenic properties of fosfomycin (FOM). The results obtained were as follows.
1) Detection of degradation products of FOM: stored solution of FOM under various pH conditions and serum including FOM were measured by gel filtration, SIMS and HPLC methods. Degradation products were not observed in any test using this experiment.
2) Formation of FOM polymer: Stored FOM solution made by GRANT's method was measured by gel filtration and HPLC technique. The gel filtration pattern of the stored solution showed diffuse elution peaks between the Kay. 0.1 and 0.5 positions. The polymer fraction of FOM was therefore not clarified.
3) Non-specific adsorption of FOM to the dialysis membrane: The dialysis effect of FOM was compared with that of PCG using the countercurrent dialyzer method.
The PCG solution was completely dialyzed in 90 minutes. The dialysis speed of FOM, however, was slower and only 50% of FOM was dialyzed in 2 or 3 days.
Our results suggest that if antigenicity of FOM is expressed, its structure is derived from carrier protein to which FOM is adsorbed.
