EFFECT OF CEFBUPERAZONE (CBPZ) ON IMMUNE BACTERICIDAL ACTION IN MOUSE

Abstract

We examined the NBT reduction activity of neutrophil and lysozyme concentrations in serum of mice treated with cefbuperazone (CBPZ) after infection with E. coli (K-14). NBT reduction activity and lysozyme concentration increased from 4-8 h, and then both decreased to normal by 24 h after subcutaneous injection with 0.1 mg or more of CBPZ/mouse. The doses in infected mice proved to be the same as in normal mice. Also, CBPZ induced the same results at concentrations from 1.0-10 μg/ml in vitro, and sera showed bactericidal action for as long as 15 h later although antibiotic activity had already disappeared.
The plaque technique of CUNNINGHAM was used to demonstrate the enhancement effect of CBPZ on antibody formation. When mice were pretreated with CBPZ and inoculated with SRBC, the number of plaque-forming cells against SRBC in the spleen increased.
From these results alone it seems that CBPZ contributes to immune bactericidal action in vivo as well as in vitro.