An easy and accurate discrimination method of Ryukyu wild boar (Sus scrofa riukiuanus) by PCR-RFLP analysis using Hinf I site of D-loop region of mitochondorial DNA

Abstract

We confirmed a cross breed Inobuta in the wild boar which we captured in Okinawa resulted in sequencing of D-loop. Furthermore, in case of amplified D-loop regions digested with Hinf I, two fragments were confirmed in case of Ryukyu wild boars but one fragment were confirmed in case of the other wild boars and pigs. In conclusions, Ryukyu wild boars were genetically different from other wild boars, Inobuta and pigs. This is a useful technique for distinction between Ryukyu wild boars and the others.