牛の射精精液中のNeuraminidase様物質について

吉田 文男; 高嶺 浩

doi:10.2508/chikusan.35.282

Studies on Neuraminidase-like Substance in Ejaculated Bull Semen

Fumio YOSHIDA, Hiroshi TAKAMINE

Author information

JOURNAL FREE ACCESS

1964 Volume 35 Issue 5 Pages 282-289

DOI https://doi.org/10.2508/chikusan.35.282

Details

Published: November 25, 1964 Received: April 14, 1964 Available on J-STAGE: March 10, 2008 Accepted: - Advance online publication: - Revised: -
Correction information
Date of correction: March 10, 2008 Reason for correction: - Correction: PDF FILE Details: -

Date of correction: March 10, 2008 Reason for correction: - Correction: PDF FILE Details: -

Date of correction: March 10, 2008 Reason for correction: - Correction: ABSTRACT Details: Wrong : It has previously been shown by the authors that mucopolysaccharide in cervical mucus of cow contains considerable amounts of sialic acid. The present investigations were initiated to study biochemical relations between this sialomucopolysacchride (SMP for short) in cervical mucus and ejaculated bull semen. The following experiments were carried out for this purpose.
Aqueous solution of SMP isolated from cervical mucus of cow was incubated with a small volume of ejaculated bull semen for a definite periods at 37°C. After removing spermatozoa by centrifugation, the supernatant fluid was dialyzed against running tap water for 2 days, then the sialic acid and galactose contents in the nondialyzable fractions were determined.
The results obtained are summarized as follows :
1) It was found that bound-type sialic acid content in the nondialyzable fraction incubated with intact semen was much less than that in the nondialyzable fraction incubated with the
semen previously heated for 30 minutes at 58°C.
2) The results essentially similar to those shown above were obtained also in experiments in which spermatozoa were used in place of semen.
3) The rate of decrease of the sialic acid content in the nondialyzable fraction incubated with the intact spermatozoa plus the seminal plasma which was heated for 30 minutes at 58°C was considerably higher as compared with that incubated with the spermatozoa which was heated for 30 minutes at 58°C plus the intact seminal plasma.
4) In each experiment mentioned above, the content in the nondialyzable fraction of the bound-type galactose, which is one of the other components in the SMP, hardly differed in the sample as compared with it in the control.
5) No interrelation was obtained between hyaluronidase and the bound- type sialic acid reducing factors found in the intact semen or spermatozoa.
6) The rate of decrease of the bound-type sialic acid content in the experiments with intact semen seemed to has positive correlation to the grade of sperm concentration and sperm activity to certain extent, but further studies will be necessary about this point.
7) From the above results, it was suggested that an enzyme-like substance, which specifically splits sialic acid bond in SMP and consequently releases only sialic acid, i.e. some substance resembling to "neuraminidase", which has been discovered hitherto only in some virus and in culture filtrates of some bacteria, might exist in ejaculated bull semen, probably in spermatozoa. But the conclusive evidence of this point must further be studied in detail.
Right : It has previously been shown by the authors that mucopolysaccharide in cervical mucus of cow contains considerable amounts of sialic acid. The present investigations were initiated to study biochemical relations between this sialomucopolysacchride (SMP for short) in cervical mucus and ejaculated bull semen. The following experiments were carried out for this purpose.
Aqueous solution of SMP isolated from cervical mucus of cow was incubated with a small volume of ejaculated bull semen for a definite periods at 37°C. After removing spermatozoa by centrifugation, the supernatant fluid was dialyzed against running tap water for 2 days, then the sialic acid and galactose contents in the nondialyzable fractions were determined.
The results obtained are summarized as follows :
1) It was found that bound-type sialic acid content in the nondialyzable fraction incubated with intact semen was much less than that in the nondialyzable fraction incubated with the semen previously heated for 30 minutes at 58°C.
2) The results essentially similar to those shown above were obtained also in experiments in which spermatozoa were used in place of semen.
3) The rate of decrease of the sialic acid content in the nondialyzable fraction incubated with the intact spermatozoa plus the seminal plasma which was heated for 30 minutes at 58°C was considerably higher as compared with that incubated with the spermatozoa which was heated for 30 minutes at 58°C plus the intact seminal plasma.
4) In each experiment mentioned above, the content in the nondialyzable fraction of the bound-type galactose, which is one of the other components in the SMP, hardly differed in the sample as compared with it in the control.
5) No interrelation was obtained between hyaluronidase and the bound- type sialic acid reducing factors found in the intact semen or spermatozoa.
6) The rate of decrease of the bound-type sialic acid content in the experiments with intact semen seemed to has positive correlation to the grade of sperm concentration and sperm activity to certain extent, but further studies will be necessary about this point.
7) From the above results, it was suggested that an enzyme-like substance, which specifically splits sialic acid bond in SMP and consequently releases only sialic acid, i.e. some substance resembling to "neuraminidase", which has been discovered hitherto only in some virus and in culture filtrates of some bacteria, might exist in ejaculated bull semen, probably in spermatozoa. But the conclusive evidence of this point must further be studied in detail.

Date of correction: March 10, 2008 Reason for correction: - Correction: PDF FILE Details: -

Abstract

It has previously been shown by the authors that mucopolysaccharide in cervical mucus of cow contains considerable amounts of sialic acid. The present investigations were initiated to study biochemical relations between this sialomucopolysacchride (SMP for short) in cervical mucus and ejaculated bull semen. The following experiments were carried out for this purpose.
Aqueous solution of SMP isolated from cervical mucus of cow was incubated with a small volume of ejaculated bull semen for a definite periods at 37°C. After removing spermatozoa by centrifugation, the supernatant fluid was dialyzed against running tap water for 2 days, then the sialic acid and galactose contents in the nondialyzable fractions were determined.
The results obtained are summarized as follows :
1) It was found that bound-type sialic acid content in the nondialyzable fraction incubated with intact semen was much less than that in the nondialyzable fraction incubated with the semen previously heated for 30 minutes at 58°C.
2) The results essentially similar to those shown above were obtained also in experiments in which spermatozoa were used in place of semen.
3) The rate of decrease of the sialic acid content in the nondialyzable fraction incubated with the intact spermatozoa plus the seminal plasma which was heated for 30 minutes at 58°C was considerably higher as compared with that incubated with the spermatozoa which was heated for 30 minutes at 58°C plus the intact seminal plasma.
4) In each experiment mentioned above, the content in the nondialyzable fraction of the bound-type galactose, which is one of the other components in the SMP, hardly differed in the sample as compared with it in the control.
5) No interrelation was obtained between hyaluronidase and the bound- type sialic acid reducing factors found in the intact semen or spermatozoa.
6) The rate of decrease of the bound-type sialic acid content in the experiments with intact semen seemed to has positive correlation to the grade of sperm concentration and sperm activity to certain extent, but further studies will be necessary about this point.
7) From the above results, it was suggested that an enzyme-like substance, which specifically splits sialic acid bond in SMP and consequently releases only sialic acid, i.e. some substance resembling to "neuraminidase", which has been discovered hitherto only in some virus and in culture filtrates of some bacteria, might exist in ejaculated bull semen, probably in spermatozoa. But the conclusive evidence of this point must further be studied in detail.

Corresponding author

Correction information

Register with J-STAGE for free!